Maxi plasmid dna kit pm010, pm025 the geneaid maxiprep plasmid kit was designed for plasmid dna purification using prepacked anionexchange resin maxiprep plasmid columns to purify plasmid dna from 2001600 ml of cultured bacterial cells. Timerun, 500g yield, high yields of highcopy plasmid dna, for purification of. The mini column has a dna binding capacity of 50 g. Endofree maxi plasmid kit is based on alkaline lysis technology followed by adsorption of dna onto silica membrane in the presence of high salt, and uses buffer p4 and filtration cs1 to wipe off the endotoxin and protein impurity effectively. Maxi the overall principle combines modified alkaline. Timerun, 500g yield, high yields of highcopy plasmid dna, for purification of up to. Qiagen endofree plasmid maxi kit, 100l to 250ml culture volume, anionexchange technology, transfectiongrade plasmid or cosmid dna purification, manual processing, 150 min. Toxout endofree plasmid midi kit k28 biovision, inc.
Invitrogen plasmid maxiprep kits offer a range options to eliminate the challenges. Endofree plasmid midi kit and the endofree plasmid maxi kit combine the power of hibind technology with omega bioteks innovative endotoxin removal technology etr to deliver highquality plasmid dna with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Qiagen plasmid maxi kit 100, 12165 from qiagen labsave. The zymopureendozero plasmid maxiprep kit features a simple spincolumn based method for the purification of up to 1. The monarch plasmid miniprep kit is a rapid and reliable method for the purification of high quality plasmid dna. The kits for pcr cleanup, gel purification, and column based plasmid kits are pretty similar in the final portion of the protocols, but differ in the sample handling early on, primarily in releasing the dna and adjusting chemistry so that it binds. Easypreptm endofree plasmid table of contents miniprep ii. Qiagen plasmid purification handbook sierra bermeja. Qiagen plasmid maxi kit, 100 preps, 100 to 500ml culture volume, transfectiongrade plasmid or cosmid dna purification, anionexchange technology, 500g yield, 160 min. Endofree plasmid maxi kit 10, 12362 from qiagen labsave.
Endofree maxi plasmid kit handbook 2 introduction endofree maxi plasmid kit is based on alkaline lysis technology followed by adsorption of dna onto silica membrane in the presence of high salt, and uses buffer p4 and filtration column cs to wipe off the endotoxin and protein impurity effectively. May 09, 2012 plasmid dna extraction using the presto midi plasmid kit from geneaid. The eluted plasmid dna is endozero and ready for immediate use in the most sensitive applications. Plasmid or cosmid dna purification using qiagen plasmid mini kit. For qiagen plasmid midi, maxi, mega, and giga kit protocols. Endofree ezflow plasmid isolation biomiga headquarters. Plasmid miniprep kit plasmid miniprep kit norgens plasmid miniprep kit is designed for the rapid preparation of plasmid dna from small batch cultures of escherichia coli. Trueblue lysis buffer an optional color indicator is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. Following lysis, endotoxins are removed from the cleared lysate with the addition of etr solution. Endotoxinfree plasmid dna from 50150 ml of bacterial. Description the accuprep nanoplus plasmid mini, midi and maxi extraction kit was developed for the extraction of highly purified plasmid dna from cultured bacterial cells within 10 min. This system applies salt free reagent to get high purity plasmid, no salt remain, and the concentration of endotoxin below 0. More than 400 g of plasmid dna is quantified with a spectrophotometer.
Qiafilter cartridges, provided in qiafilter, hispeed, and endofree plasmid kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. For transfection of sensitive cell lines endotoxins are a major pr. Identification of the preparationsubstance and of the company preparation name. The plasmid miniprep kit is able to purify plasmids up to,000 bp in. Faqs this kit is designed for fast and efficient purification of plasmid dna from 1 to 5 ml of e. The endofree plasmid miniprep kit provides an efficient endotoxin removal step into the traditional purification procedure to produce transfection grade plasmid dna. For preparation of transfectiongrade plasmid dna from e. If you are performing a midi prep to purify plasmid dna you will find volumes or incubation. The quality of the fast ion plasmid maxi kit is tested on a lottolot basis by isolating plasmid dna from a 100 ml overnight e. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Thermo scientific genejet endofree plasmid maxiprep kit is a fast, convenient, and efficient system for the large scale purification of endotoxinfree plasmid dna. Plasmid kits addgenes repository contains over 80,000 plasmids, which includes over 90 kits. Thermo scientific genejet plasmid miniprep kit utilizes an exclusive silicabased membrane technology in the form of a convenient spin column. All centrifugation steps should be carried out at 16,000 x g,000 rpm.
Plasmid maxiprep kit catalogue number of the product. Highspeed plasmid mini kit fast and economical plasmid minipreps the highspeed plasmid mini kit is designed for the rapid isolation of plasmid or cosmid dna from 14 ml of bacterial cultures. Omega biotek provides various methods for plasmid dna extraction from bacterial culturesplasmid purification kits are intended for molecular biology applications, and each plasmid kit is tested against predetermined specifications to ensure consistent product quality. Qiagen plasmid purification handbook qiagen plasmid midi, maxi, mega, and giga kits qiafilter plasmid midi, maxi, mega, and giga kits endofree plasmid maxi, mega, and giga kits for purification of ultrapure plasmid dna second edition august 2003. The kit recovers up to 20 g of high copy plasmid dna per isolation procedure. The purified plasmid 1 g is used in ecor i digestion, and checked by.
The table below will help you choose the right plasmid dna maxiprep kit. The isolation procedure is based on a modified alkaline lysis protocol and can. Plasmid dna prepared by endofree plasmid kit is suitable for a variety of routine. Endofree plasmid purification handbook 112005 7 safety information when working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. Endotoxins are major contaminants found in plasmid dna preparations. Plasmid maxi prep kit catalogue number of the product. This kit is designed for fast and efficient purification of plasmid dna from 3 to 12 ml of e. The endofree plasmid maxi kits provide an efficient method for the largescale isolation of endotoxinfree plasmid dna. Plasmid dna extraction using the presto midi plasmid kit from geneaid.
The zymopure ii plasmid midiprep kit features a simple spincolumn based method for the purification of up to 400 g of transfection grade plasmid dna in less than 20 minutes. The genopure plasmid maxi kit is used to prepare purified plasmid dna in. Endofree plasmid maxiprep kit syringe format notice. Endofree elution buffer, rnase a, ezbindtm columns10, user manual1. For more information, please consult the appropriate material safety data sheets msdss. I am trying to nucleofectelectroporate my t cell clones.
This protocol is designed for purification of up to 500 g endotoxinfree. All other materials should be stored at room temperature. These are available online in convenient and compact pdf format at. Solution ii must be tightly capped when not in use.
This document contains confidential and proprietary information. Plasmid dna mini kit i and plasmid dna mini kit ii components are guaranteed for at least 12 months from the date of purchase when stored as follows. These are available online in convenient and compact pdf format. The geneaid midi plasmid kit uses prepacked anionexchange resin columns to purify plasmid dna from 50300 ml of cultured bacterial cells. Plasmid or cosmid dna purification using hispeed plasmid midi and maxi kits. Plasmid dna purification using qiagen plasmid plus maxi kits 22 plasmid dna purification using qiagen plasmid plus mega or. For purification of up to 10 mg transfectiongrade plasmid or cosmid dna. Endofree plasmid maxi kit 10 from qiagen selectscience. Plasmid dna maxiprep kits thermo fisher scientific ru. Highspeed plasmid mini kit 300 300 preps pd300 highspeed plasmid mini kit 100 100 preps pd100 product name package size cat. What is the best plasmid extraction kit for large plasmids. Plasmid dna is then further purified by adsorbing to the hibinda silica membrane. The system utilizes lysate clearance filter syringes instead of centrifugation to rapidly clear bacterial lysates postalkaline lysis.
As an added convenience, the zymopure ii plasmid midiprep kit contains. The plasmid dna is preferentially purified from other cellular components such as genomic dna and rna. Qiagen plasmid plus purification handbook 042012 5 qiagen plasmid plus kit maxi 25 maxi 100 catalog. Check buffer p2 for sds precipitation due to low storage temperatures. Sample preparation aurum plasmid mini kit supports any downstream research with 99% plasmid dna purity requires pdf 50kb english format file size language. Use one vial of rnase a centrifuge briefly before use per bottle of buffer p1, to give a final concentration of 100. Easypreptm endofree plasmid table of contents miniprep ii manual. The eluted plasmid dna is endozero and ready for immediate use in. Highlights efficienthigh yields of up to 20 g of high quality plasmid dna fastpr. Endofree plasmid mini kit i endofree plasmid mini kit ii endofree plasmid midi kit endofree plasmid maxi kit 15 ml 515 ml 2550 ml 30 g 70 g 250 g.
Plasmid purification using a qiagen hispeed plasmid maxi kit. When transferred into filter syringelet stand the lysate at room, temperature for 1 to 2 min until the white precipitates float to the top, then press the plunger. Do not copy or distribute without prior, written permission. The zymopure ii plasmid maxiprep kit features a simple spincolumn based method for the purification of up to 1. This system applies salt free reagent to get high pure plasimd, no salt remain, and the concentration of endotoxin below 0. Solution i once rnase a is added should be stored at 28. This protocol is designed for purification of up to 500 g endotoxinfree plasmid dna using the endofree plasmid maxi kit. These are available online in convenient and compact pdf. Chargeswitch pro filter plasmid maxi kit thermo fisher scientific. To help you find what youre looking for, weve gathered our plasmid kits on one page. Hispeed plasmid midi and maxi kits protocol before staring make sure that rnase a has been added to p1 buffer.
For larger sample amount per reaction, please refer to fastion endofree plasmid maxi kit. For purification of up to 10 mg endotoxinfree advanced transfectiongrade plasmid or cosmid dna. These clones seem to be very sensitive to endotoxin in the plasmid preparation. Endofree maxi plasmid kit handbook 2 introduction endofree maxi plasmid kit is based on alkaline lysis technology followed by adsorption of dna onto silica membrane in the presence of high salt, and uses buffer p4 and filtration column cs to wipe off the. Endofree plasmid maxi kit en print bookmark share pdf 50kb english format file size language download get adobe reader contact qiagen. Each procedural step in the purification protocol is arranged like the following. The tonkbio endofree plasmid midi kit is designed for efficient and convenient extraction of endofree dna. Accuprep nanoplus plasmid extraction kit technical manual i. There is a difference between genomic dna preparation kits and plasmid preparation kits. The chargeswitch pro filter plasmid maxi kit includes 10 plasmid maxipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol. Endofree plasmid kits include an integrated endotoxinremoval step to yield plasmid dna containing plasmid dna. The geneaid maxiprep plasmid kit was designed for plasmid dna purification using prepacked anionexchange resin maxiprep plasmid columns to purify plasmid dna from 2001600 ml of cultured bacterial cells.
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